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KMID : 0379120000280020112
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Korean Journal of Mycology
2000 Volume.28 No. 2 p.112 ~ p.117
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Genetic Relationships of Internal Transcribed Spacer (ITS) Regions on Entomopathogenic Fungi by RFLP
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Lee Wang-Hyu
Choi Joung-Sik
Choi In-Young
Yu Young-Jin
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Abstract
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A similarity coefficient were analyzed by RFLP of fourteen species of entomopathogenic fungi, isolated from inhabiting pupa and adult insect at forest. Each rDNA ITS I and ITS II with primers of ITS 1 and ITS 4 was amplified by PCR. The amplified products were conserved to 500 by were not demarcated between genus and species. Four Paeciliomyces tenuipes, two Beauveria bassiana and six Cordyceps militaris were treated by seven restriction enzymes and confirmed in species except JB3 by electrophoresis band. However, the band of C. scarabaeicola showed the identity with B. bassiana. The result of this experiment indicated that the teleomorph of C. scarabaeicola was the same as that of B. bassiana. Cfo I and Hpa II of seven restricted enzymes were easily discriminating in the genus between Paecilomyces and Cordyceps. Especially, Cfo I was more effective to classify the genera of Paecilomyces, Cordyceps and Beauveria than other restriction enzymes. The hand patterns of RFLP of P. tenuipes, C. militaris, C. scarabaeicola and B. bassiana were also analyzed by UPGMA program of NTSYS-pc and showed 100% significance. Thus, the similarity coefficient tended to be lower between genera by RFLP analysis, but was higher between species.
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KEYWORD
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Beauveria bassiana, Cordyceps militaris, Entomopathogen, ITS, Paecilomyces tenuipes, RFLP
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